With reference to " uncle's Jie Shi Bacteria Identification handbook (the 8th edition) and " common bacteria system identification handbook " is carried out Physiology and biochemistry and identified change klebsiella (Klebsiella variicola) HUB-IV-005 of dwelling: the change klebsiella HUB-IV-005 starch hydrolysis experiment of dwelling is positive The fat splitting test is negative The gelatin hydrolysis test is negative Semi-solid puncture is negative HĢThe S test is negative, and urea test is positive, and indole test is negative, and methyl red test is negative, and the Fu Pu test is positive, and citrate test is positive, and the urine enzyme test is positive Decomposing sucrose produces sour aerogenesis, decomposes maltose and produces sour aerogenesis, and decomposing D-sorbyl alcohol produces sour aerogenesis, decomposes rhamnosyl and produces sour aerogenesis, decomposes N.F,USP MANNITOL and produces sour aerogenesis, reduces lactose and produces sour aerogenesis, decomposes trehalose and produces sour aerogenesis.The optimum growth temperature that becomes the klebsiella HUB-IV-005 of dwelling is 37 ℃, and the pH of right growing environment is 7.0 ± 0.2.Įmbodiment two: this embodiment becomes klebsiella (Klebsiella variicola) HUB-IV-005 of dwelling and screens in the termite enteron aisle and obtain.Screening is carried out according to the following steps: one, preparation termite homogenate: the termite of collecting 10 health in the ant nest Using volumetric concentration is that 75% ethanol is to termite sterilization 3~4min After using aseptic water washing 3 times again, cut cephalothorax then, belly is placed sterilized culture dish Afterwards, under aseptic condition, the bellies of 10 termites changed in the mortar of phosphate buffered saline buffer of the sterilized 0.1mmol/L of being equipped with pH 7.0, grind to form homogenate, add the 10mL sterilized water and be diluted to muddy liquid, be termite homogenate Simultaneously, sterile termite belly in counting the wiping of rolling on the culture medium flat plate, is put into incubator and cultivated, whether thorough with the surface sterilization of check termite Two, enrichment culture: draw the homogenate of 1mL termite with the liquid-transfering gun of sterilization and pack in the triangular flask of the 250mL that fills 30mL enrichment medium (nitrogen-free agar), cultivate 3~5d in 100r/min, 30 ℃, the nutrient solution after the enrichment culture Three, the single bacterium colony of plate isolation: with the nutrient solution gradient dilution to 10 after the enrichment culture Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Heilongjiang University filed Critical Heilongjiang University Priority to CN 201110318877 priority Critical patent/CN102344898B/en Publication of CN102344898A publication Critical patent/CN102344898A/en Application granted granted Critical Publication of CN102344898B publication Critical patent/CN102344898B/en Status Expired - Fee Related legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee Heilongjiang University Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor 赵凯 张小燕 郝妍 常志威 孙立庆 夏昕 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number CN2011103188771A Other languages Chinese ( zh) Google Patents CN102344898A - Klebsiella variicola strain CN102344898A - Klebsiella variicola strain
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